The present invention discloses an herbal hand-wash composition and method of preparation thereof. The composition consists of a synergistic mixture of Eucalyptus oil (0.1-0.25 %,, optimally 0.25 %), Pine oil- (0.1-0.25 %, optimally 0.25 %), Cardamom oil- (0.1-0.25 %, optimally 0.25 %), sodium laryal ether sulphate (10-20 %, optimally, 20 %), Coco Diethanolamide (1-2 %, optimally, 2 %) and Coco amido propyl betaine (4-5 %, optimally 5 %), Tween 80 (3-4 %, optimally, 4%), NaCl (1-2.5 %, optimally, 4%) and ethanol (4-5 %, optimally, 5%). The composition is antimicrobial, non-toxic readily available, cheaper, safer and provides medicinal benefits to the skin. There is no need to add natural color and fragrance to the hand-wash composition. The composition is made in an eco-friendly manner as two of the essential oils used in the present composition are extracted from agro-waste i.e. Pine and Eucalyptus leaves.